The Opportunity:

Revolution Medicines is seeking a highly motivated Senior Research Associate to join our Cell Culture Team and support early discovery programs. This is a hands-on laboratory role focused on generating high-quality cellular materials to enable critical go/no-go decisions. The ideal candidate is detail-oriented, collaborative, and thrives in a fast-paced discovery environment.

As the team and function grow, this role can offer meaningful opportunities for professional development and expanded responsibilities.

Key Responsibilities:

• Perform routine mammalian cell culture, including expansion, maintenance, cryopreservation, and thawing of multiple cell lines.

• Maintain accurate cell line inventory and experimental documentation using Benchling.

• Respond to urgent, time-sensitive requests from discovery teams to support key program milestones.

• Develop protocols and document experimental workflows and observations with high rigor.

• Partner closely with cross-functional teams to assess cell line needs and deliver materials on defined timelines.

• Contribute to a culture of transparent, proactive communication across teams.

• Identify opportunities to improve efficiency, robustness, and scalability of cell culture workflows.

Required Skills, Experience and Education:

• S./M.S. degree in Molecular Biology, Cell Biology, Cancer Biology, or related life science field with 3+ years of hands-on mammalian cell culture experience; biopharmaceutical or biotechnology industry experience preferred.

• Demonstrated expertise in aseptic technique and mammalian cell culture, including:

• Concurrent handling of multiple cell lines.

• Media preparation with complex supplements.

• DMSO dilution calculations and cryopreservation best practices.

• Strong experience optimizing and troubleshooting mammalian cell culture systems.

• Familiarity with cell culture quality control practices (e.g., mycoplasma testing, cell line authentication).

• Proficiency with electronic lab notebooks (e.g., Benchling) for experiment documentation, inventory management, and workflow execution.

• Excellent organizational skills with meticulous attention to detail and data integrity.

• Strong written and verbal communication skills; ability to work independently while collaborating effectively across teams.

• Ability to prioritize work, meet deadlines, and remain composed in a dynamic, fast-paced environment.

• Familiarity with standard laboratory equipment (microscopes, pipettes, centrifuges, cell counters).

• A positive, flexible, and collaborative mindset with a continuous improvement mentality.

Preferred Skills:

• Experience working with cancer cell lines, primary cells, or genetically engineered cell lines.

• Experience with viral and non-viral transduction or transfection methods (e.g., lentivirus, retrovirus, lipid-based systems).

• Hands-on experience with 2D and 3D cell culture systems, including spheroids and/or organoid models.

• Exposure to cell-based assay development and downstream applications (e.g., viability, proliferation, reporter assays).

• Experience supporting early discovery or translational research programs.

• Experience in regulated or semi-regulated laboratory environments with strong documentation standards.

• Exposure to automation, high-throughput cell culture, or scale-up workflows.

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