What’s the opportunity? 

LifeMine Therapeutics is seeking a highly motivated Scientist to join our Discovery Biology team located in Watertown, MA. We are seeking an experienced and self-directed Senior Scientist to build proteomics capabilities within our research organization. This is a hands-on, lab-based role for a scientist who thrives at the intersection of chemical biology, mass spectrometry, and mechanistic biology. The ideal candidate brings deep technical expertise in proteomics paired with strong biological intuition. You are comfortable working independently from end-to-end pre-clinical drug discovery to presenting mechanistic data to leadership. You take ownership of your platform and your science, and you are energized by the challenge of building something new and making it work in service of meaningful drug discovery. 

Key Responsibilities: 

• Propose study designs and conduct experiments to evaluate immunomodulatory response mechanisms in vitro and in vivo. Interpret data in the context of disease biology, signaling pathways, and therapeutic hypotheses; translate findings into actionable insights for cross-functional teams. 

• Design and execute bottom-up proteomics workflows including sample preparation (protein digestion, fractionation, enrichment), LC-MS/MS acquisition, and data analysis using tools such as MaxQuant, DIA-NN, Spectronaut, and Skyline  

• Perform affinity-based pulldown and co-immunoprecipitation experiments coupled with MS to identify protein-protein interactors and characterize target interactomes.  

• Execute CETSA (Cellular Thermal Shift Assay) and related target engagement assays to assess drug-target interaction in cellular contexts  

• Apply chemical biology tools including activity-based probes, photoaffinity labeling, and chemically modified ligands to support mechanism-of-action studies. 

• Partner closely with biology and translational teams to design experiments that address key program questions and efficiently contribute to clinical studies. 

• Communicate results clearly through presentations, reports, and scientific discussions with internal and external stakeholders. 

• Stay current with advances in proteomics technology, chemical biology, and relevant disease biology; bring new methodologies into the group proactively. 

What you will need to be successful: 

• Ph.D. in Biochemistry, Chemical Biology, Proteomics, Analytical Chemistry, or a related field  

• 4+ years of postdoctoral or industry experience with a strong focus on mass spectrometry-based proteomics  

• Proven hands-on experience with LC-MS/MS instrument operation (orbitrap, tribrid and timsTOF), method development, and troubleshooting  

• Demonstrated experience in bottom-up proteomics workflows (global, PTM enrichment) from sample preparation through data analysis  

• Experience with affinity pulldown/AP-MS or interactome profiling experiments is a must 

• Familiarity with CETSA or other thermal proteome profiling (TPP) approaches  

• Proficient using chemical biology tools for target identification and MoA studies  

• Strong biological understanding with the ability to contextualize proteomic findings within cellular and disease biology  

• Track record of independent experimental design, execution, and troubleshooting  

• Excellent communication skills; ability to present complex data to diverse audiences