About Treeline

Treeline Biosciences is an innovative biotechnology company, founded by an experienced team of biopharmaceutical scientists and executives, committed to developing new medicines against difficult to drug targets in cancer and other life-threatening diseases. We are pursuing a uniquely integrated approach that applies modern and emerging tools in genomics, lead discovery, medicinal chemistry, and computation to reduce the time and risks of drug discovery.

About the role

We are seeking an experienced, enthusiastic, and highly motivated Scientist to join our Watertown, MA structural biology group to support the team’s delivery of high-quality scientific data that drive small molecule drug discovery. The successful candidate will have a critical role in supporting Treeline’s drug discovery programs by collaborating closely with the multi-disciplinary Lead Discovery organization across biophysics, mass spec, biochemistry, structural biology, and protein production, as well as the broader organization including biology, computational chemistry, and medicinal chemistry. The ideal candidate will take a lead on lab equipment operation/maintenance, execute on structural experiments, and effectively communicate results to colleagues within Lead Discovery and project teams.

Responsibilities

• Establish protein crystallization conditions for small molecule soaks/co-crystallization and solve high resolution crystal structures to drive structure-based drug design.
• Maintain day-to-day operation of the structural biology laboratory and equipment.
• Provide insights and guidance to build the capabilities of the structural biology platform.
• In collaboration with project teams, establish SAR to rapidly drive the hit finding, hit-to-lead, and lead optimization process.

Qualifications

• PhD in Structural Biology, Biophysics, Biochemistry, Chemistry, Chemical Biology or related discipline with 0+ years or BS/MS with 6+ years experience in biotech/pharma industry.
• Training in crystallographic workflow: screening, crystal harvesting, ligand complexing, data collection and processing, and structure refinement.
• Experience with a Linux cloud computation environment – knowledge of databases and Python/shell scripting is a plus.
• Ability to maintain and operate sensitive lab equipment, like liquid handlers, automated imagers, and microscopes.
• Ability to work efficiently and communicate detailed results within defined timelines and to collaborate in a multidisciplinary team.
• Experience with fragment screening or novel crystallographic workflows is a plus.
• Additional experience with protein purification, biophysical methods (SPR, DSF, MST, ITC), or other structural characterization technique (cryoEM/NMR/SAXS) is a plus.

This position is classified as exempt. The anticipated annual base salary range for candidates who will work in Watertown is $126,087 to $139,666. The final base salary offered to the successful candidate will be dependent upon several factors that may include but are not limited to the type and length of experience within the job, education, and other factors. Treeline Biosciences is a multi-state employer, and this salary range may not reflect positions that work in other cities or states.

Recruitment fraud statement

Please be aware of recruitment fraud and related job scams, where scammers present themselves as recruiters but are seeking to steal money or personal information. Keep the following in mind to protect yourself:

• Treeline Biosciences will never ask for money from you as part of our recruitment process. Do not provide bank details or pay somebody for the promise of a job at Treeline.
• We do not conduct interviews through Skype or Telegram.
• Our job openings are first posted to treeline.bio/careers. If you locate a listing for a Treeline job on another site, please ensure it is also posted on our careers website.
• You can find more information about job scams at consumer.ftc.gov/articles/job-scams.
• To report job scams, head to ReportFraud.ftc.gov.

Scientist, NMR & Biophysics

About the Role

We are seeking a highly motivated Biophysics Scientist to join our Watertown team to deliver high-quality results and drive small molecule drug discovery. In this role, you will be a key contributor to our small‑molecule discovery engine, designing and executing biophysical strategies that identify high‑quality hits, elucidate mechanisms of action, and accelerate lead optimization. You will work at the center of multidisciplinary project teams, partnering closely with chemistry, biology, and lead discovery to advance programs toward development candidates. The successful candidate will design and implement new biophysical assays and hit ID approaches with a focus on ligand observed and protein observed NMR, along with other techniques such as SPR and DSF.  You will provide expert interpretation of biophysical results and use data analysis and visualization tools to integrate data with the broader set of data generated at Treeline to drive program decisions.

Responsibilities

• Design, develop, and execute biophysical assays to support hit identification, hit-to-lead, and lead optimization activities.
• Establish robust NMR assays to monitor small molecule binding
• Efficiently utilize a suite of biophysical and biochemical techniques to advance drug discovery
• Effectively analyze, interpret, and communicate biophysical data as a member of project teams.
• Employ biophysical methods to mechanistically deconvolute the molecular mechanism of action of small molecule hits.
• In collaboration with chemistry, biology, lead discovery, and medicine design, characterize and establish SAR to rapidly drive the hit-to-lead process.

Required Qualifications

• • PhD in Biophysics, Biochemistry, Chemistry, Chemical Biology or related discipline with 0+ years of relevant postdoctoral training or Masters with 6+ years.
  • Biotech/pharma experience with a demonstrated impact in hit identification, hit-to-lead, and lead optimization for small-molecule programs a plus.
  • Expertise in quantitative biophysical assay development, especially NMR for small‑molecule drug discovery. Hands-on experience characterizing protein–ligand interactions, using both ligand observed and protein observed experiments
  • Experience with programming (python) and implementing ML workflows a plus
  • Demonstrated ability to troubleshoot complex assays, work with challenging proteins, and deliver high‑quality, reproducible data.
  • Excellent communication, data visualization, and interpersonal skills, with a track record of effective collaboration on multidisciplinary teams.

Preferred Qualifications

• Experience with fragment-based lead discovery, covalent lead discovery, and/or DEL
• Experience with implementing ML workflows and programming
• Experience with 19F fragment-based NMR screening
• Theoretical and practical knowledge of an array of biophysical and biochemical methods with proven hands-on expertise applying biophysics for interaction analysis. Expertise with MST/SS, DSF, MS, SPR, TR-FRET and/or FP assays.
• Working knowledge of adjacent disciplines (biochemistry, structural biology, protein purification, biology, chemistry).
• Experience in working with challenging proteins.
• Experience working with CRO’s

This position is classified as exempt. The anticipated annual base salary range for candidates who will work in Watertown is $126,087 to $139,666. The final base salary offered to the successful candidate will be dependent upon several factors that may include but are not limited to the type and length of experience within the job, education, and other factors. Treeline Biosciences is a multi-state employer, and this salary range may not reflect positions that work in other cities or states.

Recruitment fraud statement

Please be aware of recruitment fraud and related job scams, where scammers present themselves as recruiters but are seeking to steal money or personal information. Keep the following in mind to protect yourself:

• Treeline Biosciences will never ask for money from you as part of our recruitment process. Do not provide bank details or pay somebody for the promise of a job at Treeline.
• We do not conduct interviews through RingCentral, Skype or Telegram. 
• Our job openings are first posted to treeline.bio/careers. If you locate a listing for a Treeline job on another site, please ensure it is also posted on our careers website.
• You can find more information about job scams at consumer.ftc.gov/articles/job-scams.
• To report job scams, head to ReportFraud.ftc.gov.

Senior Scientist Biophysics

About the Role

We are seeking an experienced, self-starter, and highly motivated Biophysics Senior Scientists to join our San Diego team to deliver high-quality results and drive small molecule drug discovery. In this role, you will be a key contributor to our small‑molecule discovery engine, designing and executing biophysical strategies that identify high‑quality hits, elucidate mechanisms of action, and accelerate lead optimization. You will work at the center of multidisciplinary project teams, partnering closely with chemistry, biology, and lead discovery to advance programs toward development candidates. The successful candidate will propose, design, and implement new biophysical assays and hit ID approaches (ie. SPR, MST, DSF, NMR), characterize protein-small molecule and protein-protein interactions, and uncover mechanistic insights of our drug discovery targets and lead molecules. You will provide expert interpretation of biophysical results and use data analysis and visualization tools to integrate data with the broader set of data generated at Treeline to drive program decisions.

Responsibilities

• Propose, design, develop, and execute biophysical assays to support hit identification, hit-to-lead, and lead optimization activities.
• Establish robust SPR assays for small‑molecule interaction analysis, including hit triage, affinity/kinetic characterization, and mechanism-of-action studies.
• Efficiently utilize a suite of biophysical techniques to advance drug discovery: primarily SPR but also including DSF, DLS, and others through in-house and CRO capabilities.
• Employ biophysical methods to mechanistically deconvolute the molecular mechanism of action of small molecule hits.
• Effectively analyze, interpret, and communicate biophysical data as a subject matter expert and biophysics representative on project teams.
• In collaboration with chemistry, biology, lead discovery, and medicine design, characterize and establish SAR to rapidly drive the hit-to-lead process.
• Propose and evaluate new approaches and technologies.
• Provide scientific leadership, mentorship, and cross-functional partnership within a dynamic matrixed environment.

Required Qualifications

• PhD in Biophysics, Biochemistry, Chemistry, Chemical Biology or related discipline with 2-3 years of relevant postdoctoral training.
• 4+ years of biotech/pharma experience with a demonstrated impact in hit identification, hit-to-lead, and lead optimization for small-molecule programs.
• Expertise in quantitative biophysical assay development, especially SPR for small‑molecule drug discovery. Hands-on experience characterizing protein–ligand interactions, including affinity, kinetics, stoichiometry, and mechanism.
• Strong theoretical and practical knowledge of an array of biophysical methods with proven hands-on expertise applying biophysics for interaction analysis. Expertise with MST/SS, DSF, MS, or NMR are a plus.
• Demonstrated ability to troubleshoot complex assays, work with challenging proteins, and deliver high‑quality, reproducible data.
• Excellent communication, data visualization, and interpersonal skills, with a track record of effective collaboration on multidisciplinary teams.

Preferred Qualifications

• Ability to lead in a matrix environment, and for example, act as point of contact on project teams.
• Experience with fragment-based lead discovery, covalent lead discovery, and DEL are a plus.
• Strong hands-on biochemistry experience or expertise in enzymology.
• Working knowledge of adjacent disciplines (biochemistry, structural biology, protein purification, biology, chemistry).
• Experience in working with challenging proteins.
• Experience working with CROs.

This position is classified as exempt. The anticipated annual base salary range for candidates who will work in San Diego is $151,694 to $174,363. The final base salary offered to the successful candidate will be dependent upon several factors that may include but are not limited to the type and length of experience within the job, education, and other factors. Treeline Biosciences is a multi-state employer, and this salary range may not reflect positions that work in other cities or states.

Recruitment fraud statement

Please be aware of recruitment fraud and related job scams, where scammers present themselves as recruiters but are seeking to steal money or personal information. Keep the following in mind to protect yourself:

• Treeline Biosciences will never ask for money from you as part of our recruitment process. Do not provide bank details or pay somebody for the promise of a job at Treeline.
• We do not conduct interviews through RingCentral, Skype or Telegram. 
• Our job openings are first posted to treeline.bio/careers. If you locate a listing for a Treeline job on another site, please ensure it is also posted on our careers website.
• You can find more information about job scams at consumer.ftc.gov/articles/job-scams.
• To report job scams, head to ReportFraud.ftc.gov.